NAD+ & NADH kit (RUO)

Key Features

• Highly accurate and sensitive. Quantitation limit is 330 nM for NAD+ and 119 nM NADH in whole blood measured in two 96-well plate assays.
• Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
• High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.

Principals

The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a blood sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.

Reagents Provided   

Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, Assay BUFFER C, Assay color reagent, Enzyme, Stop solution, Positive control

Kit Requires 

Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader

Precautions and Warnings

For research use only by trained personnel.
The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.

More details how to prepare samples in the sample preparation document – vs.2, last modified September 2023.