NAD+ & NADH Tissue/Cell kit (RUO)
Quantitative assay kit for tissues and cells
| Catalog number / name | RUO_003 / Q-NAD Tissue/Cell NAD+ and NADH assay kit |
| Detection method | Colorimetric (OD570-573nm) |
| Assay type | Quantitative |
| Samples type | Tissues / Cells |
| Species | All |
| Guideline for sample volume | 20 mg of tissue per 1 mL of extraction buffer 0.5 M cells per 100 μL of extraction buffer |
| Metabolites measured | NAD+, NADH |
| Detection Limit | 25 nM for NAD+ and 35 nM NADH in the cell/tissue extract |
| Assay Length | < 1h |
| Size | 192 reactions |
| Number of samples | 40 (measured in duplicates) |
| Storage | Store reagents at -80°C |
| Intended use | RUO |
| Shelf Life | 6 months |
Instructions for use, Vs. 4, Last updated September 2024
Key Features
- Highly accurate and sensitive.
- Easy to handle. One step metabolite extraction is followed by separate measurement of NAD+ and NADH in the extract. Detection time is less than 10 minutes.
- High throughput. Can be easily adopted into high-throughput 96-well plate assay to analyze hundreds of samples per day.
Principals
The principle of the assay is a cyclic enzymatic reaction with a colorimetric end-point detection. First, NAD+ and NADH metabolites are extracted together from a tissue or cell culture sample in a single step. Then the extract is divided into two parts revealing both NAD+ and NADH concentrations by removing one metabolite and stabilizing the other.
Reagents Provided
Extraction BUFFER A, NAD+ stabilizing reagent, NADH stabilizing reagent, NAD+ standard stock, NADH standard stock, BUFFER C,
BUFFER D, Assay color reagent, Enzyme, Stop solution, Positive control
Kit Requires
Multi-channel pipets. Two clear-bottom 96-well plates suitable for colorimetric assays, heat block with adjustable temperature (up to 80°C), spectrophotometric plate reader
Precautions and Warnings
For research use only.
The Stop Solution may cause skin, eye, and respiratory irritation. Avoid breathing in fumes.
Assay color reagent may cause skin irritation. Handle with care, use gloves.
BUFFER A can cause eye irritation. Handle with care, use googles.
Read more in the Safety Data Sheet (SDS) – vs.4, last modified January 2024
General Information
Proprietary name: Q-NAD tissue/cells: quantitative assay kit for NAD+ and NADH in tissues and cells
Catalog number: RUO_003, 40 samples (96-well format), 192 reactions
More details how to prepare samples in sample preparation document. Protocol for cell collection with NADMED lysis buffer you can find here (vs. 2, Last update April 2024).