FAQ

 

What are NADs?

NADs (nicotinamide adenine dinucleotides) are coenzymes which are essential to metabolism. NADs are found in every living cell. There are four different types of NAD: NAD+, NADH, NADP+ and NADPH.

See more Unlock the secrets of NADs

How to purchase NADMED kits and services?

Fill in the contact form and we’ll get in touch with you. We do not provide the kit or our services to individuals.

However, if NAD testing of indivdual samples is what you are looking for turn to your local clinic or laboratory. Hopefully they have NADMED method in use.

 

What equipment is needed to run NADMED assay?

This is a list of necessary items and equipment you need to perform a NADMED assay:

• Deionized water (milli-Q water from a water purification system or commercially available deionized water, e.g., Sigma cat #38796) 
• Microtubes, 1.5 mL, for sample preparation, extraction, stabilization, and assay standards preparation. Requirements for the material of the microtubes: use basic non-sterile microcentrifuge tubes made from transparent/natural color polypropylene (PP) intended for in vitro diagnostics (e.g., Sarstedt Ref 72.690.001). Do not use microtubes intended for molecular biology labeled as sterile (chemically sterilized), free of endotoxin, pyrogen, human DNA, and low retention; do not use microtubes intended for work with proteins labeled as LoBind. 
• Two plastic multichannel pipette reservoirs (from non-sterile polystyrene): one for pipetting the master mix, the other for pipetting the Stop solution. 
• Two 96-well transparent polystyrene microplates with medium protein binding affinity intended for colorimetric and absorbance assays. 
• Dry bath (heat block) with adjustable temperature (up to 80°C). 
• Table-top cooling microcentrifuge (max speed 20 000 x g). 
• Ice-water bath (packed ice with added tap water to a slush state, which firmly holds inserted microtubes, preventing them from floating). 
• Calibrated single channel pipettes (0.5–10 μL, 5–50 μL, 20–200 μL, 100–1000 μL) and multichannel pipettes (5–50 μL, 30–300 μL) and beveled pipette tips, low retention. 
• Spectrophotometric microplate reader capable of measuring absorbance at 570–573 nm. 
• Aluminum foil to protect microtubes and plates from light

 

What is the difference between NADMED and other NAD measuring kits?

The current NAD measuring kits are not accurate enough to provide results that can guide e.g. NAD boosters dosage in proper order. See more “Groundbreaking technology”.

How to prepare samples?

Whole blood (human) 

– Blood should be collected into the smallest EDTA vacutainer (2 ml)
-IMPORTANT! If possible, the blood sample should be divided into 150 – 200 μL aliquots in separate test tubes, e.g. Eppendorf tube
-Samples should be frozen at -20 °C within 4-6h after withdrawal for several days. Samples should be stored for longer time in -80 °C
IMPORTANT! Keep consistent time between withdrawal and freezing for all samples

Whole blood (animal)

-Blood should be collected into the EDTA tubes and divided into 150 – 200 μL aliquots in separate test tubes, e.g. Eppendorf tube
-Samples should be frozen at -20°C as soon as possible after withdrawal for several days.
-Samples should be stored for longer time in -80°C
IMPORTANT! Keep consistent time between withdrawal and freezing for all samples
Read more

 

How to store the kit upon arrival?

In -20°C freezer with consistent temperature.

How many samples can be measured with one kit?

40 samples measured in duplicates, each metabolite (e.g. NAD+ and NADH) are measured on separate 96 well plates.

What samples can be used for the NAD measurements?

NADMED method can utilize whole blood, tissues and cultured cell (human or animal). Most NADs are found in cells – plasma and serum contain very low levels of NADs and are not suitable for NADMED technology.

For more details, please contact us

What is the optimal sample size?

We recommend using 150 – 200μL/sample of whole blood, approx 20 mg/sample of tissue, and 0.5 – 1M/sample of cultured cells. For more details, please contact us.

Where to find instructions for use?

Instruction can be found on our website in section “documents”.

 

Can the kit be used several times?

No, the kit is for one time use. We do not recommend freezing / thawing the kit components.

How do you report the NAD levels?
  • Blood: NAD levels are normalized per volume (final concentration is in μM)
  • Tissues/cells: NAD levels are normalized per sample weight or protein amount